The present study performs an experimental measurement on transient thermal response of an air-conditioned space by a variable air volume (VAV) system with a PI(pro-portional-integral) control logic. A thermal chamber with a PI controlled VAV unit is constructed to verify the previously suggested stratified multi-zone model. The effects of thermal parameters and control parameters such as supply air temperature and PI control factor are investigated by implementing the thermal chamber test. The experimental results obtained show that transient behavior of the air-conditioned space-temperature is in good accordance with the simulation results of the stratified thermal model.