The mechanical properties of carbon fiber and glass fiber reinforced PEEK hybrid composites with different starting sequence were evaluated through tensile, flexural, interlaminar shear strength (ILSS) and impact test. Tensile and flexural strength of hybrid composites increased with the fiber volume fraction. Interlaminar shear strength was affected by stacking sequence of the hybridization. In the impact test, the hybrid composites with glass fiber ply at impact side absorbed more energy and showed synergy effect in impact energy absorption.