Differential pulse voltammetry (DPV) using nafion-coated glassy carbon electrodes modified with Tetren(tetraethylene pentamine)-glycerol showed sensitivity for determining lead (II) at low concentration. The Lead (II) was accumulated on the electrode surface by the formation of the complex in an open circuit, and the resulting surface was characterized by medium exchange, electrochemical reduction, and differential pulse voltammetry. Various experimental parameters, such as the composition of modifier, preconcentration time, pH of electrolyte (0.1 M acetate buffer), and parameters of differential pulse voltammetry, were optimized. The initial potential was applied for 50 s, the electrode was scanned from -0.9 to -0.3 V, and the anodic peak current was measured at -0.604 V $pm$ 0.015 V (vs. Ag/AgCl). The calibration plot was obtained in the range 1.0$ imes$10$^{-8}$ M~l.0$ imes$10$^{-6}$ M with pH 4.5 buffer solution. The detection limit (3$sigma$) it as low as 5.0$ imes$ 10$^{-9}$ M. This method is applied to the determination of lead(II) in a certified reference material and the result agrees satisfactorily with the certified value.