The Holographic Particle Velocimetry system can be a promising optical tool for the measurements of three dimensional particle velocities. In this study, the holographic particle velocimetry system was used to measure the sizes and velocities of droplets produced by a commercial full cone spray nozzle. As a preliminary validation experiment, the velocities of glass beads on a rotating disk were measured with uncertainty analysis to identify the sources of all relevant errors and to evaluate their magnitude. The error of the particle velocity measured by the holographic method was 0.75 ㎧, which was 4.5% of the known velocity estimated by the rotating speed of disk. The spray droplet velocities ranged from 10.3 to 13.3 ㎧ with average uncertainty of ${pm}$ 1.6 ㎧, which was ${pm}$ 14% of the mean droplet velocity. Compared with relatively small uncertainty of velocity components in the normal direction to the optical axis, uncertainty of the optical axis component was very high. This is due to the long depth of field of droplet images in the optical axis, which is inherent feature of holographic system using forward-scattering object wave of particles.