Femtosecond laser ablation mechanism was systematically investigated on sodalime glass in ambient conditions. The ablation crater diameter was measured for varying numbers of laser pulses as for varying well as the laser fluence. The analysis of the results with a one dimensional spatial Gaussian fluence distribution reveals that the inherent ablation mechanism has been altered from a multi-photon process to a single photon excitation due to defect sites that have been accumulated by successive laser pulses. Furthermore, the transition between the two regimes was found to be a function of both the laser fluence and the number of laser shots.