This paper deals with speed control of DC servo motor using a high gain obserber. It was designed to estimate rotor speed of DC servo motor and it carries out speed control from the feedback of the estimated speed signal. Also, PI controller was used in speed controller. In order to verify the performance of the high gain observer which is proposed in this paper, it is compared estimate performance of Luenberger Observer and High Gain Observer with the computer simulation. Effectiveness of the proposed high gain observer is proved from the experiment to compare the case with a speed sensor to the case with high gain observer in the speed control of DC servo motor.