An experimental investigation was performed to study the characteristics of turbulent water flow in a horizontal circular tube by using liquid crystal. To determine some characteristics of the turbulent flow, 2D PIV technique is employed for velocity measurement and liquid crystal is used for heat transfer experiments in water. Temperature visualization was made quantitatively by calibrating the color of the liquid crystal versus temperature using various approaches (TLC technique: Thermochromic Liquid Crystal), and a neural-network algorithm was applied to the color-to-temperature calibration. This study shoud the temperature and time-mean velocity distribution for Re = 2,436, 2,500 and 2,724 along longitudinal sections and the results appear to be physically reasonable.