Nitrification efficiency of fixed film biofilters with sand, loess bead, and styrofoam bead in biofilter columns of 1-m height and 30 cm width was studied. Synthetic wastewater was continuously supplied to the culture tank to maintain total ammonia nitrogen (TAN) concentration in the inflow water at around 8 mg/L. The hydraulic loading rate was set at 200 ㎥/$m^2$/day. TAN conversion was stabilized after about 90 day conditioning for all the selected filter media but with net accumulations of nitrite. On the volumetric basis, conversion rates of TAN and nitrite were the highest in styrofoam bead filter. Mean volumetric TAN conversion rates in the final samples were 682, 269, and 79 g TAN/㎥/day in the styrofoam bead, sand and loess bead filters, respectively. Low gravity and cost of styrofoam bead render the handling easier and more cost-effective.