This study was carried out to investigate pathogenicity of liquid spawns on dongchunghacho growing using silkworm, optimum infective condition for mass production and infection rate on various conservation periods and temperatures. We compared with the infection rates after conservation the liquid spawns (Paecilomyces tenuipes) at 4$^{circ}C$ and 25$^{circ}C$ for 10 days and 20 days. In these results, infection rate of conservated liquid spawns at 4$^{circ}C$ was 88.0 percent in the tenth day, and then again it was 5.3 percent at 25$^{circ}C$. Infection rates at 4$^{circ}C$ were on the whole excellent out of long-term preservation methods.