Livestock manure have been utilized as fertilizer, and trying to make resources natural circulation of organic materials. This experiment was carried out to investigate the effect of swine slurry on production and nutritive value of rye, and to determine optimal application level for 2 years from Oct. 2000 to May 2002 in the experimental field of Grassland and Forage Crops Division, National Livestock Research Institute. Summary of the results were as follows. The application of swine slurry 200% Plot showed the best regrowth after winter and lodging was severe in all plots except non-fertilized plot. Plant height was lower in lodging plots. The dry matter yield was not significantly difference between chemical fertilizer(CF) plot and swine slurry application plots, and swine slurry 100% + CF 50% plot showed the highest yield among manure application plots. The dry matter yield per input nitrogen was 80kg in CF, 81kg in swine slurry 100% application plot. Above the level, it was decreased severely by 41 ∼ 61kg. Conclusively, application to swine slurry 100% showed superior productivity considering the DM yield per input nitrogen, That level would be recommended to produce higher yield and to conserve environment.