This paper deals with speed control of DC servo motor using a Adaptive high gain obserber. In this parer, the gain of the observer is properly set up using the fuzzy control and adaptive high gain observer that have a superior transient characteristic and is easy to implement compared the existing method is designed. In order to verify the performance of the Adaptive high gain observer which is proposed in this paper, it is compared estimate performance of High-gain Observer and Adaptive High Gain Observer with the computer simulation. Effectiveness of the proposed high gain observer is proved from the experiment to compare the case with a speed sensor to the case with Adaptive high gain observer in the speed control of DC servo motor.