Extensive studies have been conducted for reducing the residual stresses and birefringence in injection-molded optical disk substrate Flow-induced and thermally-induced stresses and birefringence have been found as two main sources during injection molding process. However, high speed rotation also induces extra stresses and birefringence in real operation of disk drives. In the present paper rotation-induced in-plane birefringence has been measured and presented for CD and DVD substrates at different radial position. About 10 - 15 nm of extra retardation in one pass has been measured up to 4,800 rpm. The distribution of extra rotation-induced birefringence will be valuable data for designing an optimal optical disk substrate. Finally, experimental results were compared with the extra stresses calculated from simple formulation.