This study was carried out to develop a vacuum nozzle seeder for the automation of large seeds sowing of fruit vegetables and rootstocks. Moreover, the seeding efficiency was examined to find the optimum operating condition considering high precision seeding. The important operating factors for high seeding rate were typically nozzle diameter and absorbing vacuum pressure. The optimum nozzle diameters were found 1.5, 1.5 and 2.0 mm for Chambak, Tuktozwa and Hukjong while the optimum vacuum pressures were 8.0㎪, 10.6㎪ and 5.3㎪, respectively. Under the optimum operating condition, the results indicated that the maximum seeding rates were 97.6％, 98.8％ and 97.6％ respectively for Chambak Tuktozwa and Hukjong. The vibrating acceleration of the hopper did not make any significant effects on the seeding rate when the vacuum pressure reached 8.0㎪ and the sowing rate became higher with lighter seed. As the seed became heavier, the larger diameter of nozzle was recommended 1.5mm of the nozzle diameter was found to be applied for the experimental seeds. The vacuum pressure was also found 8.0㎪ - 13.3㎪ at that time.