As concrete structures are getting larger, higher, longer, and specialized, it is more required to develop steel fiber concrete and apply to the real world. In this research, it is aimed to have fatigue strength examined, varying the steel fiber content by 0%, 0.75%, 1.00%, 1.25%, by experimental study of Two-spans Beam with Steel Fibrous with repeated loads. The ultimate load and the initial load of flexural cracking were measured by static test. In addition, the load versus strain relation, load versus strain relation, load versus deflection relation, crack pattern and fracture mode by increasing weight was observed. On the other hand, the crack propagation and the modes of fracture according to cycle number and the relation of cycle loading to deflection relation and strain relation was observed by fatigue test. As the result of fatigue test, Two-spans Beam without Steel Fibrous was failed at 60∼70% of the static ultimate strength and it could be concluded that fatigue strength to two million cycle was around 67.2% by S-N curve. On the other hand, that with Steel Fibrous was failed at 65∼85% of the static ultimate strength and it could be concluded fatigue strength to two million cycle around 71.7%.