For the construction of open-topped steel box girder bridges, prefabricated concrete slab could offer several advantages over cast-in-situ deck including good quality control, fast construction, and elimination of the formwork for concrete slab casting. However, precast decks without reinforcements at transverse joints between precast slabs should be designed to prevent the initiation of cracking at the joints, because the performance of the joint is especially crucial for the integrity of a structural system. Several prestressing methods are available to introduce proper compression at the joints, such as internal tendons, external tendons and support lowering after shear connection. In this paper, experimental results from a continuous composite bridge model with precast decks are presented. Internal tendons and external tendons were used to prevent cracking at the joints. Judging from the tests, precast decks in negative moment regions have the whole contribution to the flexural stiffness of composite section under service loads if appropriate prestressing is introduced. The validity of the calculation of a cracking load fur serviceability was presented by comparing an observed cracking load and the calculated value. Flexural behavior of the continuous composite beam with external prestressing before and after cracking was discussed by using the deflection and strain data.