The purpose of this experimental study is to investigate the relationship of the shear behavior and the variety of width, depth and location of an opening in reinforced concrete deep beams with rectangular web openings, and to present an improved shear strength equation of those members. The main parameters considered were concrete strength(fck), shear span-to-overall depth ratio(a/h), and the size and vortical position of the web openings. Twenty five deep beams were tested under two symmetric loading-points. Test results showed that the shear behavior of deep beams with web openings was influenced by a/h and the size of opening. In addition, the KCI shear design provision is a tendency to be more unconservative according to the increase in a/h and the area-ratio of opening to shear span(Ao/Ash). Based on the concrete strut action of top and bottom member of an opening and the tie action of longitudinal reinforcement, a proper design equation which closely predicts the capacity of deep beams with rectangular openings is developed.