Urolithiasis (UL) is a common disease of canine. Analysis of urinary stone is an essential step in the examination and initial treatment of the patient with UL. The dead maned wolf (Chrysocyon brachyurus), male, was introduced to the Veterinary Teaching Hospital, Chungbuk National University. The maned wolf was imported from USA to Cheong-ju zoo. Postmortem sign of maned wolf was hemorrhage of bladder with a many stones. No bacteria was shown with bacterial culture of urine. But in feces, Clostridium perfringens were overgrown. Bladder stone from maned wolf was composed of calcium oxalate. Calcium oxalate was formed with excess calcium from diet mainly meat base.