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Synthesis and Characterization of a Receptor-Targeting Contrast Agent
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  • Synthesis and Characterization of a Receptor-Targeting Contrast Agent
  • Synthesis and Characterization of a Receptor-Targeting Contrast Agent
저자명
Yang. Taegyun,Park. Ji-Hyung,Lee. Seung-Cheol,Kim. Cheol-Su,Cho. Jee-Hyun,Lee. Chul-Hyun,Cheong. Chae-Joon
간행물명
Journal of the Korean magnetic resonance society
권/호정보
2003년|7권 1호|pp.46-54 (9 pages)
발행정보
한국자기공명학회
파일정보
정기간행물|ENG|
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기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

We synthesized a contrast agent for MRI that is capable of binding to the ABP-1 receptor and enhancing the contrast of the targeted cells. We used a lysine dendrimer (G=3)DTPA[Gd] as the contrast agent and synthesized a biotinylated polyclonal antibody for ABP-1 as the first antibody. Lysine dendrimers were prepared using the solid phase peptide synthesis method.$^3$ Amino-terminated lysine dendrimers were then coupled to DTPA using the anhydride method. Gd was complexed with the DTPA-lysine dendrimer in an acidic solution of 3 eq GdCl$_3$ to one of DTPA. The lysine dendrimer-DTPA[Gd] and avidin were conjugated in MES solution, pH 6.0, using EDC as the coupling reagent. The biotin-avidin system was used to link the polyclonal antibody and contrast agent. K562 cells were used for imaging.