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A Comparative Study on the Protective Role of Trehalose and LEA Proteins against Abiotic Stresses in Transgenic Chinese Cabbage(Brassica campestris) Overexpressing CaLEA or otsA
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  • A Comparative Study on the Protective Role of Trehalose and LEA Proteins against Abiotic Stresses in Transgenic Chinese Cabbage(Brassica campestris) Overexpressing CaLEA or otsA
  • A Comparative Study on the Protective Role of Trehalose and LEA Proteins against Abiotic Stresses in Transgenic Chinese Cabbage(Brassica campestris) Overexpressing CaLEA or otsA
저자명
Park. Seong-Hee,Jun. Sung-Soo,An. Gyn-Heung,Hong. Young-Nam,Park. Min-Chul
간행물명
Journal of plant biology
권/호정보
2003년|46권 4호|pp.277-286 (10 pages)
발행정보
한국식물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Trehalose and LEA proteins, representative low MW chemicals that are synthesized under dehydration, are known to protect plants from drought stress. To compare their effectiveness on enhancing tolerance against various abiotic stresses, we generated transgenic Chinese cabbage plants overexpressing E. coli trehalose-6-phosphate synthase gene (otsA) or hot pepper (Capsicum annuum) LEA protein gene (CaLEA). Both transgenic plants exhibited altered phenotype including stunted growth and aberrant root development. When subjected to drought, salt or heat stress, these plants showed remarkably improved tolerance against those stresses compared with nontransformants. After dehydration treatment, leaf turgidity and fresh weight was better maintained in both transgenic plants. CaLEA-plants performed somewhat better under dehydrated condition. When treated with 250 mM NaCl, both otsA-plants and CaLEA-plants remained equally healthier than nontransformants in maintaining leaf turgidity and delaying necrosis. Furthermore, leaf Chl content and Fv/Fm was maintained considerably higher in both transgenic plants than nontransformants. After heat-treatment at 45$^{circ}C$, both transgenic plants appeared much less damaged in external shape and PSII function, but LEA proteins were more protective. Our results indicate that although both trehalose and LEA proteins are effective in protecting plants against various abiotic stresses, LEA proteins seem to be more promising in generating stress-tolerant transgenic plants.