The purpose of this study is the effect of massage therapy on the human autonomic nerve system by heart rate variability spectral analysis. This study were to effect autonomic nerve system before and after massage therapy in 12 health peoples. The effect of massage therapy were assessments through Heart Rate Variability (HRV; SA-2000E, Medicare, Korea) before and after the massage therapy by same massage therapist. Statistical techniques for data analysis were applied paired t-test. The 0.05 level of significance was used as the critical level for rejection of the null hypotheses for the study. On the basis of the results analyzed in this study, conclusions were drawn as follows; Rest Heart Rate was significantly decreased before and after massage therapy, but other variable were no statistical significances. These results suggest that massage therapy maybe be effective in reducing heart rate and activity in valgal tone.