Purpose: This study analyzed the effects of a CD- ROM educational program for the prevention of sexual violence in relation to the sexual knowledge of middle school students. Method: A one group, pre-test-post-test design was used. The subjects of this study were 171 middle school students in Kangwon Province, Korea. The questionnaire included 34 items relating to overall sexual knowledge, and included questions on sexual violence. The collected data were analyzed using the mean, standard deviation and paired- t tests. Result: There were significant differences in the sexual knowledge by demographic characteristics between the pre- and post- tests. Of the 34 items, 20 showed significant differences in the sexual knowledge between the pre- and post- tests. Conclus ion: The overall level of sexual knowledge of the students increased after taking the CD- ROM educational program. Further testing will be required to determine the effects of the CD- ROM using a control group and random sampling.