The purpose of this research was to investigate inhibitory activity of Sasang medicines on diabetes. Reduce 36 kind of medicines to powder, abstract add 100 times methanol. Examine inhibitory activity on α-amylase and α-glucosidase in vitro. Thirty six oriental herbs were screened for inhibitory activities against carbohydrate digesting enzymes in vitro. Methanol extract of Pleuropterus multiflorus Turez, Ephedra sinica, Moutan cortex Radicis, Magnolia obovata, Alpinia officinarum, Amomi Semen, Rubus coreanus, and Cinnamomi cortex inhibited yeast α-glucosidase activity and extract of Moutan cortex Radicis and Rubus coreanus inhibited porcine α-amylase activity by more 50%. Rubus coreanus showed the strongest inhibition against both α-glucosidase and α-amylase. Therefore, Rubus coreanus was the most effective medicice of 36 kind of medicines.