This paper reports a series of component tests on a lightweight floor system and a method to predict the natural frequency of the floor using a frame analysis program. Full-scale floor tests are also briefly described. DuraGal steel Rectangular Hollow Sections (in-line galvanised RHS) are used as joists, bearers and piers in DuraGal lightweight floor systems. A structural grade particleboard is used as decking. Connection stiffness between different components (bearer, joist, pier and floor decking) was determined. A 40% composite action was achieved between the RHS joist and the particleboard. Both 2D and 3D models were developed to study the effect of connection stiffness on predicting the natural frequency of DuraGal lightweight floor systems. It has been found that the degree of shear connection between the bearer and the joist has a significant influence on the floor natural frequency. The predicted natural frequencies are compared with measured values from full scale floor testing.