Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate mixture $(lidocaine;HCL/NaHCO_3)$ and tricaine methanesulfonate (MS-222) was tested for the greenling (Hexagyammos otakii) at three different temperature regimes: $12^{circ}C,;8^{circ}C;and;24^{circ}C.$ Based on the exposure and recovery time, effective dose of lidocaine $HCl/NaHCO_3$ was 800 ppm $(18^{circ}C);and;300;ppm;(24^{circ}C)$ for greenling of $21.0pm1.4;cm$ body length. Anaesthetic dose and temperature-dependent relationship in exposure and recovery time were observed. Effective dose of MS-222 at $18^{circ}C$ was proven to be 125 ppm and 150 ppm. Combination of lidocaine $HCl/NaHCO_3$ and MS-222, considerably reduced the dosage of each anaesthetic required to give rapid, deep anaesthetic condition. In the dry exposure after anaesthetic, the control fish exhibition $22\%$ mortality after dry exposure of 20 min; whereas, the anaesthetic condition with 800 ppm lidocaine $HCl/NaHCO_3$ for 1 min exhibited delayed recoveries from the anaesthetic condition with mortalities of $20\%,;41\%,;78\%;and;100\%$ after dry exposures of 8 min, 12 min, 16 min and 20 min, respectively. The results indicate that lidocaine $HCl/NaHCO_3$ can be used as suitable anaesthetic for the greenling.