The present studies have been designed to evaluate the effect of acute wound healing that frequency and intensity of pulsed ultrasound application in diabetic rat. Mild diabetes mellitus was induced in rat with 45 mg/kg streptozotocin. The results were analyzed and summarized as follows: 1. The rate of wound length on ultrasonic capacity in $1.0;W/cm^2$ $SATA_i$ group was more significantly decreased than $0.5;W/cm^2$ $SATA_i$ group. 2. In the histological change, the $1.0;W/cm^2$ $SATA_i$ application group was more effective than $0.5;W/cm^2$ $SATA_i$ group, decrease of inflammatory cell was significantly in several groups. From the conclusions above, in this study application of 3 MHz pulsed ultrasound in acute wound healing, $1.0;W/cm^2$ $SATA_i$ capacity can be an effective way of promotion wound healing than $0.5;W/cm^2$ $SATA_i$ capacity in diabetic rat.