Purpose: This study was done to investigate the relationship between fatigue and exercise in middle-aged women. Method: A descriptive survey study design was used. Result: The fatigue level for middle-aged women was 1.98. It was found that about 52.7% of the participant do exercise now. Fatigue according to exercise level was not significant, but the degree of fatigue was low for women who do exercise. Conclusion: The degree of fatigue for middle-aged woman should be assessed and exercise suited to each person should be taught. Fatigue needs to be controlled before it develops to the point of disease, and appropriate exercise is an suitable intervention.