The purpose of this study was to design the method of 3D space calibration to reduce RMSE by statistical analysis when using the DLT algorithm and control frame. Control frame for 3D space calibration was consist of $1{ imes}3{ imes}2m$ and 162 contort points adhere to it. For calculate of 3D coordination used two methods about 2D coordination on image frame, 2D coordinate on each image frame and mean coordination. The methods of statistical analysis used one-way ANOVA and T-test. Significant level was ${alpha}=.05$. The compose of methods for reduce RMSE were as follow. 1. Use the control frame composed of 24-44 control points arranged equally. 2. When photographing, locate control frame to center of image plane(image frame) o. use the lens of a few distortion. 3. When calculate of 3D coordination, use mean of 2D coordinate obtainable from all image frames.