This research has been conducted to collect regional ecotypes of Poa pratensis throughout the southern part of korean peninsula during 2000~2002. 227 local ecotypes of Poa pratensis were collected mostly from roadsides, riversides and rice paddies. The $35^{circ}$ latitude was supposed to be the southern limit of native Poa pratensis habitat. 130 ecotypes of Poa pratensis found from the poor environmental condition such as roadsides showed Poa pratensis naturally grew throughout the country. When surveyed the habitat environment, it was concluded that native Poa pratensis were mostly found on open fields or half shaded areas, on sandy soil or sandy loam soil, and in the individual patch type.