Galaxy clusters as the densest and most prominent regions within the large-scale structure can be used as well characterizable laboratories to study astrophysical processes on the largest scales. X-ray observations provide currently the best way to determine the physical properties of galaxy clusters and the environmental parameters that describe them as laboratories. We illustrate this use of galaxy clusters and the precision of our understanding of them as laboratory environments with several examples. Their application to determine the matter composition of the Universe shows good agreement with results from other methods and is therefore a good test of our understanding. We test the reliability of mass measurements and illustrate the use of X-ray diagnostics to study the dynamical state of clusters. We discuss further studies on turbulence in the cluster ICM, the interaction of central AGN with the radiatively cooling plasma in cluster cooling cores and the lessons learned from the ICM enrichment by heavy elements.