In order to determine the effect of intravenous He-Ne laser irradiation, values of serum total cholesterol, Triglyceride, LDL-cholesterol and HDL-cholesterol were measured. Twenty three patients with hyperlipidemia who visited Gunpo Oriental Medical Center of Wonkwang University were treated with He-Ne laser irridiation and analyzed by Paired T-test of SPSS program. The following results were obtained : The serum Total cholesterol and LDL-cholesterol of patients treated with He-Ne laser irradiation showed a significant(P<0.05) decrease, and the serum Triglyceride and HDL-cholesterol of patients treated with He-Ne laser irradiation showed no significant(P<0.05) effects.