Lipozyme RM IM 효소적 촉매를 사용한 glycerolysis에 의한 옥수수유로부터 $1,3-DAG(Y_1),;1,2-DAG(Y_2)$, 총 $DAG(Y_3),;MAG (Y_4)$, 및 총 DAG + MAG 의 합성 양$(Y_5)$의 생성 최적화를 반응 표면분석을 이용하여 모니터링 하였으며 반응온도$(35-75^{circ}C,;X_1)$, 반응시간$(1-11시간,;X_2)$ 및 hexane 의 혼합 양$(0-2mL,;X_3)$을 변수로 한 중심합성계획으로 최적화 실험하였다. 1,3-DAG는 반응온도$(44.92^{circ}C)$, 반응시간(10.24시간) 및 hexane 첨가량(1.16mL) 조건에서 20.43 area%로 최대값을 나타내었으며 1,2-DAG는 $56.32^{circ}C$, 6.95시간 및 1.04mL 조건에서 26.78 area% 로 최대값을 예측할 수 있었다. 총 DAC는 $53.82^{circ}C$, 8.03시간 및 1.08mL 실험 조건에서 45.09 area% 최대값을 보였다. 또한 MAG 생산 최적조건은 $64.14^{circ}C$, 7.00시간 및 0.13mL에서 최대값 9.57 area%로 예측되었으며 총 DAG+MAG 생산 최적조건은 $54.07^{circ}C$, 7.98시간, 1.02mL이었으며 이때 예측된 값은 53.54 area%이었다.
Response surface methodology was used to optimize production conditions of monoacylglycerol (MAG) and diacylglycerols (DAG) from corn oil by enzymic glycerolysis. Contents of $1,3-DAG;(Y_1),;1,2-DAG;(Y_2),;total;DAG;(Y_3),;MAG;(Y_4)$, and total $DAG+MAG;(Y_5)$ were obtained. Conditions were optimized using central composite design with incubation temperature $(35-75^{circ}C,;X_1)$, incubation time (1-11 hr, $X_2$), and amount of hexane added (0-2 mL, $X_3$) as three variables. Content of 1,3-DAG was maximized by 20.43 area% at incubation temperature of $44.92^{circ}C$, incubation time of 10.24 hr, and hexane content of 1.16 mL, whereas that of 1,2-DAG (26.78 area%) was maximized at $56.32^{circ}C$, 6.95 hr, and 1.04 mL, respectively. Predicted maximum total DAG content was 45.09 area% at $53.82^{circ}C$, 8.03 hr, and 1.08 mL, while production conditions of MAG (9.57 arae%) were $64.14^{circ}C$, 7.00 hr, and 0.13 mL. At variables of $54.07^{circ}C$, 7.98 hr, and 1.02 mL, maximum content of total DAG+MAG predicted by RSM was 53.54 area%.
