The purpose of this study was to find the effects of the eccentric exercise induced delayed muscle soreness on proprioception, muscle strength, muscle fatigue, and muscle pain of the elbow flexor muscles. Thirty one healthy male subjects were participated in this study. Before resisted eccentric exercise of the elbow flexors and immediately and at 1, 3, 5, and 7 days post-exercise, pain threshold, proprioception, tension tracking, initial median frequency, and fatigue index were measured. Pain pressure threshold and visual analog scale (VAS) was used to measure muscle pain. Proprioception of the elbow joint was measured by using 3 dimension motion analysis system. Maximum isometric contraction was measured by using digital tensiometer. Electromyography and power spectrum analysis was used to measure initial median frequency (IMF) and fatigue index (FI). Immediately post-exercise, a significant decrease pain threshold was observed that continued to 5 days post-exercise. VAS score was significantly increased at 1 and 3 days post-exercise compared to that of immediately post-exercise. Maximum isometric contraction, IMF, tension tracking ability of the exercised elbow joint were significantly decreased at 1, 3, and 5 days post-exercise compared to that of pre-exercise. FI was significantly increased at 1 and 3 days post-exercise compared that of pre-exercise. Proprioception sense of exercised elbow joint was significantly decreased immediately and at 1, 3, and 5 days post-exercise compared to that of pre-exercise. Proprioception sense of the contralateral elbow joint was significantly decreased immediately post-exercise compared to that of pre-exercise. However, proprioception sense that was measured in close chain kinematic position was not significantly difference between pre-exercise and post-exercise. These results could be useful to determine the resume time for exercising and participating sports activities.