This study investigated that the effect of rewetting agent on dentinal microtensile bond strength(${mu}TBS$). Human molars were sectioned to expose the superficial dentin surfaces. Samples were divided into two groups according to type of adhesives-Single Bond (S) and One-Step (0)], and again subdivided into five groups by different dentin surface treatment-dry for 15s (D), blot dry (BD) or dry for 15s, and rewet with different rewetting agents [distilled water (DW), Gluma Desensitizer (GD) and Aqua-Prep (AP)] for 30s. After application of adhesive, composite resin was built up on the bonding surface. Each tooth was sectioned to obtain stick with $1 extrm{mm}^2$ cross sectional area and the ${mu}TBS$ was determined by EZ test. In the S group, the mean ${mu}TBS$ of GD, AP, and BD group was significantly higher than that of DW and D group (p < 0.05), In the O group, the mean, ${mu}TBS$ of AP, GD, BD and DW group was significantly higher than that of D group (p < 0.05). The data suggested that Gluma Desensitizer and Aqua-Prep could be successfully used as rewetting agents, and Distilled water could be acceptable in aceton based adhesive system only.