A direct numerical simulation is performed to analyze the effects of a localized time-periodic blowing on a turbulent boundary layer flow at R $e_{+}$=300. Main emphasis is placed on the blowing frequency effect on near-wall turbulent flow structures at downstream. Wall-normal velocity on a spanwise slot is varied periodically at different frequencies (0.004$leq$ $f^{＋}$$leq$0.080). The amplitude of periodic blowing is $A^{＋}$=0.5 in wall nit, which corresponds to the value of $v_{rms}$ at $y^{＋}$=15 without blowing. The frequency responses are scrutinized by examining the phase or time-averaged turbulent statistics. The optimal frequency ( $f^{＋}$=0.03) is observed, where maximum increase in Reynolds shear stress, streamwise vorticity fluctuations and energy redistribution occurs. The phase-averaged stretching and tilting term are investigated to analyze the increase of streamwise vorticity fluctuations which are closely related to turbulent coherent structures. It is found that the difference between PB and SB at a high blowing frequencies is negligible.e.e.