The present agricultural water quality standards are set by a policy goal. This is intended for water quality management of public water resources, but not for the use of water resources. These standards were not determined by considering the influence of water quality on the safety of agricultural produce and the growth, yield and quality of agricultural crops. Thus, this study was carried out to investigate the influence of irrigation water quality on the growth, yield, and grain quality of rice and acquire fundamental knowledges to set up irrigation water quality standards. The pot experiment was conducted with 4 treatments using irrigation waters with various total nitrogen concentrations (control, 1, 5, 10, 20mg/L) and replicated four times with randomized block design. The results of this study showed that plant height, number of tiller, plant dry weight, the uptake of N, P, and K, and rice protein contents tended to increase as the T-N concentration in irrigation water was increased. In addition, grain yield at T-N 20 mg/L was significantly higher than in the control, but the percentage of head rice was slightly lower due to the increase of green kernel and white belly/core kernel.