Particle Image Velocimetry (PIV) measurements have been carried out to investigate the pulsatile flow characteristics in a triangular grooved channel. The results showed that a vortex was generated at the tip of the groove and flowed into the groove rotating inside during the acceleration phase of the main stream promoting the mixing of the fluid. Then, at the deceleration phase of the main stream, the vortex entrained fluid from the relatively slow moving main stream to grow bigger than the groove size. Finally the vortex was ejected to the main stream carrying the fluid away from the groove, resulting in the enhancement of mixing between the stagnant fluid in the groove and the main stream in the channel. It was found that the fluid mixing enhancement is maximized when the pulsatile period is the same as the time duration which the vortex takes to grow larger enough to fill the groove and to be ejected to the main stream.