This study investigated the effects of carnitine and/or ${gamma}$ -aminobutyric acid (GABA) supplementation on lipid profiles and some immune related nutrient in mice. Balb/c male mice were orally treated with either an AIN-76 diet (Con), a control diet plus carnitine (CS, 0.5 g/kg bw), a control diet plus GABA (GS, 0.5 g/kg bw) or a control diet plus carnitine plus GABA (CGS, 0.25 g/kg bw, respectively) for 6 weeks. There were no significant differences in feed consumption, energy intake, body weight gain or feed efficiency ratio among the groups during the experimental period. However, abdominal fat deposits were smaller in CS, GS and CGS groups compared with the Con group. Serum and liver triglycerides also were lower in CS, GS and CGS and serum total cholesterol was significantly lower in the CGS group compared with the Con group. Serum LDL cholesterol was lower in the CGS group and liver HDL cholesterol was significantly higher in the CS group compared with Con group. In serum, stearic acid and selecholeic acid were lower, but arachidic acid was higher in the CS group. Liver stearic acid was higher but oleic acid lower in CGS group compared with Con group. In carnitine supplemented groups, serum and liver nonesterified carnitine (NEC), acidsoluble acylcarnitine (ASAC), total carnitine (TCNE) concentrations were higher in only the CS group, not CGS group. Serum vitamin A and E concentrations were not different among the groups. These results may suggest that carnitine and/or GABA supplementation improves lipid profiles in mice, but did not affect the immune-related nutrients that we measured under the experimental conditions of this study.