This paper presents a sensorless speed control of a DC servo motor using a fuzzy-sliding observer in the presences of load disturbances. A fuzzy-sliding observer is proposed in order to estimate the speed of a motor rotor. First, a sliding observer is used to estimate the derivative of the armature current directly using the armature current mesured in the DC servo motor. Second, the optimal gain of the Luenberger observer is set up using the fuzzy control. Experimental results show the good performance in the DC servo motor system with the proposed fuzzy-sliding observer.