The effect of supplemental yellow $eta$-8-Apo-carotenoic acid ethyl ester (ACA) (0-300mg/kg feed) on its accumulation in each body part of laying hens and the pigmentation in egg yolk was determined. The chickens used in this study were 78-wk-old ISA brown laying hens. ACA significantly affected the color of the bodies of laying hens. The yellowness( $b^{*}$) but not the redness ( $a^{*}$) and, the lightness ( $L^{*}$) of the skin, and muscle as measured by a colorimeter significantly (P＜0.05) increased when ACA was fed at 50-100mg/kg feed. Egg yolk color was also significantly affected by feeding ACA longer than 2 days (P＜0.05) at the levels higher than 50mg/kg feed.eed.d.