This study was conducted to investigate the effects on the growth and yield by the different seeding methods and cultivating root weight in Atractylodes macrocephata Koidz. Seeding distances were different such as 5${ imes}$5 cm, 5${ imes}$10 cm, 10${ imes}$10 cm, 10${ imes}$15 cm by the hand seeder and 15 cm seeding in drill. Emergence date, plant height were not significantly changed with seeding space, but the number of leaf, fresh weight of above-ground part and fresh weight of root were increased in the sparse seeding compared with the dense seeding. The highest fresh root yield was 1,012 kg/10 a at the 5${ imes}$5 cm. Roots yield was increased in the sparse seeding compared with the dense seeding, but the highest number of roots above 16 g yield was observed at the 10${ imes}$10 cm seeding distance. Emergence date was faster 1∼3 day root weight above 6 g than that root weight 5 g. The plant height, number of stem and fresh weight of above-ground part were more increased as the root weight was heavier. The growth of underground part were more increased as the root weight was heavier, yield was increased about 27％ to 112％ compared with root weight above 6 g than that 134.6 kg/10 a with root 5 g.