The Auditory Brainstem Response (ABR) with a click stimulation in guinea pigs was used to examine the auditory neuro-pathway from the cochlear nucleus to brain. Using multi-channel active electrodes, the 3-dimensional auditory pathway was examined from the cochlea to the inferior colliculus through the brainstem. These results are similar to the well-known neuro-pathway. This study on the multi-channel ABR shows that the positions of the ABR generators move to the central brain and the contralateral pathway. It is generally agreed that the ABR is generated by some structures along the auditory pathway. This study provides some information on the neuro-pathway where the ABR peak is generated.