The end-effector is the one of the important factors on development of the cucumber robot to harvester a cucumber. Three end-effectors were designed the single blade end-effector with one blade, the double blade end-effector with two blades and the triple blade end-effector with three blades. Performance tests of the end-effector, the fully integrated system, were conducted to determine the cutting rate by using two different kinds of cucumber. The success rates of cucumber cutting ratio of single end-effector, double end-effector and triple end-effector in laboratory. were 61.7%, 95%, 86.7%, respectively. The cutting rate of single blade or double blade was a little difference with respect to the different diameters of cucumber stem. However, the success cutting rate of the end-effector with triple blade was 61.7% under 29mm diameter of a grabbing stem section. The triple end-effector was not suitable for harvesting a cucumber, but was considered to be suitable for harvesting a grape, an apple and a tomato. The success rate of cucumber cutting ratio of triple end-effectors in greenhouse was 84%. The failure cutting rate was 16% which are due to abnormal shape of cucumber fruit.