It is important to measure precisely the size and velocity of micro-bubbles used in various field. The synchrotron X-ray micro-imaging technique was employed to measure the size and velocity of micro-bubbles moving in an opaque tube simultaneously. Phase contrast images were obtained at interfaces of micro-bubbles between water and air due to their different refractive indices. The X-ray micro-imaging technique was found to measure an optical fiber with an accuracy of 0.2%. Micro-bubbles of 20∼60$mu extrm{m}$ diameter moving upward in an opaque tube (${Phi}$＝2.7mm) were tested to measure bubble size and up-rising velocity. For DI water, the measured velocity of micro-bubbles is nearly proportional to the square of bubble size, agreed well with the theoretical result. In addition, the synchrotron X-ray micro-imaging technique can measure accurately the size and velocity of several overlapped micro-bubbles.