The purpose of this study is to investigate the characteristics of thermal comfort index and to present the thermal comfort range through regression analyses and experiment in a radiant floor heating system laboratory. The results were compared to the comfort zone of ISO-7730, and the applicability of the thermal comfort index to a radiant floor heating system was studied. On comparing the sedentary posture on the floor to sitting on the chair, the comfort zone and the neutral point of comfort index showed different values. It is considered that the influence of conduction from floor to the human is sufficient. Moreover, we could find a correlation between the thermal sensation votes of subjects, and the comfort indexes were lower than those by calculation.