In this study the in vitro protective effects of several antioxidant vitamins (vitamin C, $alpha$-tocopherol, $eta$-carotene), fruits and vegetables (strawberry, tangerine, orange and 100％ orange juice, carrot juice), on the levels of isolated human lymphocyte DNA damage was measured using Comet assay. Comet assay has been used widely to assess the level of the DNA damage in the individual cells. Lymphocytes were pre-treated for 30 minutes with antioxidant vitamins (10, 50, 100, 500 $mu$M) or fruits$.$vegetables (10, 100, 500, 1000 $mu$g/ml), an4 then oxidatively challenged with 100 $mu$M $H_2O$$_2$ for 5 min at 4$^{circ}C$. The protective effect of antioxidant vitamins against DNA damage at a concentration of 50 $mu$M were 50％ in vitamin C, 32％ in $alpha$-tocopherol, whereas, fJ-carotene showed a 55％ protection at a dose as low as 10 $mu$M. The inhibitory effects of DNA damage by strawberry, tangerine, orange, orange juices, carrot juices were 50 - 60％ with wide ranges of doses. The results of the present study indicate that most the antioxidant vitamins and fruits$.$vegetables juices produced a significant reduction in oxidative DNA damage.