The inverse problem of determining unknown inlet temperature in thermally developing, hydrodynamically developed two phase laminar flow in a parallel plate duct is considered. The inlet temperature profile is determined by measuring temperature in the flow field. No prior information is needed for the functional form of the inlet temperature profile. The inverse convection problem is solved by minimizing the objective function with regularization method. The conjugate gradient method as iterative method and the Tikhonov regularization method are employed. The effects of the functional form of inlet temperature, the number of measurement points and the measurement errors are investigated. The accuracy and efficiency of these two methods are compared and discussed.