In this study, we prepared polyurethane rigid foams through a one-shot process, using 100 parts of polyols (PEG:glycerol:starch=9:1:l0 in the weight ratio), 60 to 100 parts of 2,4-TDI as an isocyanate, and 1 to 20 parts of water as a blowing agent. The foams had open-cell structure, whose cell size was approximately 0.5-1 mm and the thickness of the cell wall decreased with increasing water content. The apparent densities also decreased with increasing water content, 0.232-0.141 g/㎤ in TS5-60, 0.233-0.074 g/㎤ in TS5-80, and 0.265-0.085 g/㎤ in TS5-100, respectively. To confirm the reactions between isocyanate groups of 2,4-TDI and hydroxyl groups of polyols (PEG, glycerol, and starch) or water, FT-IR spectroscopic analysis and a solubility test in DMF were performed. The thermal property of the foams was investigated using DSC. From the results of the impact test, the impact resistance of the foams showed a maximum of 48.092 J/m at TS5-60-6, 70.951 J/m at TS5-80-8, and 88.062 J/m at TS5-100-8, respectively. From the compression test, the compressive stress showed a maximum of 402.5 KPa at TS5-60-6, 621.3 KPa at TS5-80-6, and 604.9 KPa at TS5-100-8. And the compressive elastic modulus showed a maximum of 14.94 MPa at TS5-60-6, 15.72 MPa at TS5-80-7, 16.52 MPa at TS5-100-8, respectively.