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Quantification of Oxygen Transfer in Test Tubes by Integrated Optical Sensing
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  • Quantification of Oxygen Transfer in Test Tubes by Integrated Optical Sensing
  • Quantification of Oxygen Transfer in Test Tubes by Integrated Optical Sensing
저자명
Wittmann. Christoph,Schutz. Verena,John. Gernot,Heinzle. Elmar
간행물명
Journal of microbiology and biotechnology
권/호정보
2004년|14권 5호|pp.991-995 (5 pages)
발행정보
한국미생물생명공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Immobilized sensor spots were applied for online measurement of dissolved $O_2$, in test tubes. Oxygen transport was quantified at varied shaking frequency and filling volumes. The k$_{L}$ a increased with increasing shaking frequency and decreasing filling volume. In non-baffled tubes the maximum $k_{L}a$ value was $70h^{-1}$, equivalent to a maximum $O_2$ transfer capacity of 15mMh^{-1}$. Monitoring of the hydrodynamic profile revealed that the liquid bulk rotated inside the tube with an inclined liquid surface, whereby the angle between the surface and tube wall increased with increasing shaking frequency. The $k_{L}a$ clearly correlated to the surface area. Placement of four baffles into the tubes improved the oxygen transfer up to 3-fold. The highest increase in $k_{L}a$ was observed at high filling volume and high shaking frequency. The maximum $k_{L}a$ in baffled tubes was $100 h^{-1}$.