Objectives: This study was designed to investigate effects of Gwibitang on the glutamate-induced toxicity of primary rat neonatal astrocytes. Methods and Results: Gwibitang significantly recovered the glutamate-induced apoptosis and inhibited the generation of $H_2O_2$ in astrocytes. In addition, both Gwibitang and antioxidants such as GSH reduced the glutamate-induced cytotoxicity in astrocytes, indicating that Gwibitang possibly had an antioxidative effect. Moreover, Gwibitang also inhibited the glutamate-induced degradation of Bcl-2 protein and poly(ADP)-ribose polymerase (PARP) in astrocytes. Conclusions: We suggest that Gwibitang has protective effects on glutamate-induced cytotoxicity via an antioxidative mechanism.