꽃송이버섯(Sparassis crispa DSMZ 5201)의 균사를 사용하여 균사외 효소활성을 측정하였다. Yeast-malt extract-glucose 배지를 사용하여 $24^{circ}C$에서 15일간 배양 후 배양여액을 조효소원으로 사용하였을 때, $alpha$-amylase효소의 활성은 44.27 unit/$mg{cdot}protein$이었다. 배양여액 중의 Protease, CMCase, $eta$-glucosidase, chitinase 및 exo-$eta$-1,4-glucanase의 세포외 효소활성은 상대적으로 높았으나, xylanase 효소활성은 낮게 나타났다.
The mycelia of Sparassis crispa DSMZ 5201 were cultivated at $24^{circ}C$ for 15 days in yeast-malt extract-glucose broth (pH 4.0) and the filtrate was used as crude enzyme solution to determined the extracellular enzyme activity. The specific activity of $alpha$-amylase was 44.27 unit/protein. The specific activities of protease, CMCase, $eta$-glucosidase, chitinase, exo-$eta$-l,4-glucanase were relatively high. However, a very little activity of xylanase was found.
